Description:
This is a web tool to create artificial data sets for testing pattern discovery programs. Artificial sequences are generated according to a certain nucleotide distribution with a set of embedded motifs from the ABS database. The motifs are planted in a subset of similar positions to simulate the existance of related motifs in a set of orthologous or co-regulated genes from a microarray experiment. A graphical representation of the produced data set is also displayed.

Parameters:
- Length: size of each artificial sequence (bp)
- Number of sequences: size of the data set
- Number of planted motifs: motifs included on each sequence
- Species: a specific data set to detect motifs from this species will be produced
- Nucleotide distribution: A,C,G,T content of the random regions between planted motifs
- Probability to plant a motif: probability to insert a motif in a sequence
- TF name: planted motifs are associated to these TFs

Example:
This is an artificial data set produced by this application.

Number of Sequences Number of Planted Motifs 1 2 3 4 5 6 7 8 9 10 20 50 100 1 2 3 4 5 6 7 8 9 10 20 Length (nucleotides) Probability to plant a motif 500 1000 2000 5000 0.0 0.1 0.25 0.5 0.75 0.9 1.0 Species Background composition Human Mouse Rat A C G T TF name (multiple choice) AML AP1 AP2 AP2A CAAT CEBP CETS CJUN CMYC COUP CREB CUTL E2 E2F E2F1 E47 EGR1 ETS1 FXR GATA3 GATA6 GC GR HIF1 HLTF HMGI HNF1 HNF3 HNF3B HNF4 HNF6 HOX HP1 HSF1 IPF1 IRF M1 MAZ MEF2 MEF3 MITF MTF1 MYB MYF MYOD MZF1 NF1 NFAT NFKB NFY NKX2 NRL OCT OLF1 P53 POU1F1 POU2F1 RAR RFX1 RXR SMAD SP1 SRF STAT3 TBP TEF1 USF VDR

HINTS:
1. More than one TF can be selected (multiple choice is allowed).
2. Use Shift to select groups of consecutive TFs
3. Use Ctrl to select groups of non consecutive TFs