PyHIST is a Histological Image Segmentation Tool: a lightweight semi-automatic pipeline to extract tiles with foreground content from SVS histopathology whole image slides (with experimental support for other formats). It is intended to be an easy-to-use tool to preprocess histological image data for usage in machine learning tasks.

The latest version of PyHIST, including installation steps and tutorials, can be found on the PyHIST GitHub repository. An overview of the tool is published in PLoS Computational Biology Software.