Supplementary Materials for Guigó et al (2003)
PNAS, 100(3):1140-1145 [ Abstract ]
  IMIM * UPF * CRG * GRIB HOME DATASETS Mouse/Human GP Chr15
   
CHROMOSOME 15 RT-PCR SUBMITTED GENES SUMMARY

GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
 
BR
 
HE
 
KI
 
TH
 
LI
 
ST
 
MU
 
LU
 
TE
 
SK
 
EY
 
OV
SEQ
CNF
SEQ
WRG
DIF
SPL
12D1 TS.chr15.22.004  21309754 - 21322003 FWD 318bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
7F1 TS.chr15.26.022  25956967 - 25967930 FWD 513bp / 4 2 0 0 0 0 0 0 1 1 1 1 1 1 0 1 0
3G4 TS.chr15.35.018  34574620 - 34577189 FWD 1047bp / 3 2 1 0 0 1 0 1 0 1 1 0 1 1 1 0 1
3G5 TS.chr15.36.020  35890317 - 35898302 FWD 654bp / 4 1 1 1 1 1 1 1 0 1 1 1 1 1 1 0 0
12F6 TS.chr15.46.009  45855650 - 45858447 REV 408bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12E1 TS.chr15.49.004  48212175 - 48226745 REV 837bp / 5 3 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
3G6 TS.chr15.49.011  48880626 - 48889753 REV 363bp / 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
8H1 SGP.chr15_469  50116650 - 50156502 FWD 309bp / 4 3 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
1C1 SGP.chr15_483  51540486 - 51601294 FWD 558bp / 5 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12F1 TS.chr15.59.026  58961546 - 58997112 FWD 1881bp / 15 13 0 1 0 0 0 1 1 1 1 0 0 0 1 0 0
7F2 TS.chr15.63.015  62677698 - 62683883 FWD 354bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
11E2 SGP.chr15_672  66384938 - 66424740 REV 720bp / 9 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
5E2 SGP.chr15_1159  90232752 - 90238997 REV 267bp / 2 1 0 1 0 1 0 1 0 0 1 0 1 0 1 0 0
7F3 TS.chr15.95.012  94344144 - 94348984 REV 1221bp / 2 1 0 0 0 1 1 1 1 1 1 1 1 0 0 1 0
1B6 SGP.chr15_1311  99484106 - 99502305 FWD 396bp / 4 2 1 1 1 1 0 1 0 0 1 0 1 0 1 0 0
12C1 TS.chr15.102.007  101311270 - 101324193 FWD 351bp / 4 2 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0
GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
BR HE KI TH LI ST MU LU TE SK EY OV SEQ
CNF
SEQ
WRG
DIF
SPL
 

TABLE COLUMNS DESCRIPTION

Here we describe what does contain each column in the previous table:

  • Geneva identifier: corresponds to an alphanumeric code for each single gene set of experiments.
  • Predicted gene identifier: the name of the given gene prediction as provided by the software. Both, this column and the previous one, are linked to a single browser page for each gene prediction that contains further information about it.
  • Gene location: the forward coordinates along the chromosome and the corresponding gene strand.
  • Length of the transcript and the number of exons.
  • The selected intron number.
  • Results on tissues: RT-PCR success on each of the mouse tissues tested in this experiment ("green" color meaning success and "red" color for failure). The tissues were: brain (BR), heart (HE), kidney (KI), thymus (TH), liver (LI), stomach (ST), muscle (MU), lung (LU), testis (TE), skin (SK), eye (EY) and ovary (OV).
  • Three extra columns summarizing RT-PCR results:
    • SEQ CNF : RT-PCR succeeded and the sequence of the predicted gene was confirmed.
    • SEQ WRG : the PCR product that was sequenced does not correspond to the predicted one (unspecific amplification).
    • DIF SPL : the RT-PCR determined a different splice site (i.e. an extra exon within the submitted intron).
    Real Positive Genes are those, and only those, having "1" in the "SEQ CNF" column.

 
  Disclaimer webmaster